A Pseudomonas aeruginosa-Derived Particulate Vaccine Protects against P. aeruginosa Infection.

ABSTRACT: Despite numerous efforts to develop an effective vaccine against Pseudomonas aeruginosa, no vaccine has yet been approved for human use. This study investigates the utility of the P. aeruginosa inherently produced polyhydroxyalkanaote (PHA) inclusions and associated host-cell proteins (HCP) as a particulate vaccine platform. We further engineered PHA inclusions to display epitopes derived from the outer membrane proteins OprF/OprI/AlgE (Ag) or the type III secretion system translocator PopB. PHA and engineered PHA beads induced antigen-specific humoral, cell-mediated immune responses, anti-HCP and anti-polysaccharide Psl responses in mice. Antibodies mediated opsonophagocytic killing and serotype-independent protective immunity as shown by 100% survival upon challenge

SUBMITTER: Gonzaga ZJC 

PROVIDER: S-EPMC8309987 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature