Characterization of the Domoic Acid Uptake Mechanism of the Mussel (Mytilus galloprovincialis) Digestive Gland.

ABSTRACT: Cultures of the mussel Mytilus galloprovincialis are frequently affected by accumulation of the amnesic shellfish poisoning toxin domoic acid (DA). This species is characterized by a fast uptake and release of the toxin. In this work, the main characteristics of the uptake mechanism have been studied by incubation of digestive gland thin slices in media with different composition and DA concentration. DA uptake seems to follow Michaelis-Menten kinetics, with a very high estimated K_M (1722 µg DA mL^-1) and a V_max of 71.9 µg DA g^-1 h^-1, which is similar to those found for other amino acids in invertebrates. Replacement of NaCl from the incubation media by Cl-choline (Na⁺-free medium) did not significantly reduce the upta

SUBMITTER: Blanco J 

PROVIDER: S-EPMC8310042 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature