TASPERT: Target-Specific Reverse Transcript Pools to Improve HTS Plant Virus Diagnostics.

ABSTRACT: High-throughput sequencing (HTS) is becoming the new norm of diagnostics in plant quarantine settings. HTS can be used to detect, in theory, all pathogens present in any given sample. The technique's success depends on various factors, including methods for sample management/preparation and suitable bioinformatic analysis. The Limit of Detection (LoD) of HTS for plant diagnostic tests can be higher than that of PCR, increasing the risk of false negatives in the case of low titer of the target pathogen. Several solutions have been suggested, particularly for RNA viruses, including rRNA depletion of the host, dsRNA, and siRNA extractions, which increase the relative pathogen titer in a metagenomic sample. However, these solutions are costly and time-consuming. Here we present a faster and co

SUBMITTER: Espindola AS 

PROVIDER: S-EPMC8310100 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature