Bothrops moojeni Venom and Its Components Strongly Affect Osteoclasts' Maturation and Protein Patterns.

ABSTRACT: Osteoclasts (OCs) are important for bone maintenance, calcium balance, and tissue regeneration regulation and are involved in different inflammatory diseases. Our study aimed to evaluate the effect of Bothrops moojeni's venom and its low and high molecular mass (HMM and LMM) fractions on human peripheral blood mononuclear cell (PBMC)-derived OCs' in vitro differentiation. Bothrops moojeni, a Brazilian lanced-head viper, presents a rich but not well-explored, venom composition. This venom is a potent inducer of inflammation, which can be used as a tool to investigate the inflammatory process. Human PBMCs were isolated and induced to OC differentiation following routine protocol. On the fourth day of differentiation, the venom was added at different concentrations (5, 0.5, and

SUBMITTER: D'Amelio F 

PROVIDER: S-EPMC8310197 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature