An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity.

ABSTRACT: The contribution of mitochondria to oncogenic transformation is a subject of wide interest and active study. As the field of cancer metabolism becomes more complex, the goal of targeting mitochondria using various compounds that inflict mitochondrial damage (so-called mitocans) is becoming quite popular. Unfortunately, many existing cytotoxicity assays, such as those based on tetrazolium salts or resazurin require functional mitochondrial enzymes for their performance. The damage inflicted by compounds that target mitochondria often compromises the accuracy of these assays. Here, we describe a modified protocol based on differential staining with two fluorescent dyes, one of which is cell-permeant (Hoechst 33342) and the other of which is not (propidium iodide). The difference in staining

SUBMITTER: Pei J 

PROVIDER: S-EPMC8310432 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature