Stability of SARS-CoV-2 IgG in multiple laboratory conditions and blood sample types.

ABSTRACT:

Background

COVID-19 seroprevalence studies use serum/plasma samples to detect SARS-CoV-2 IgG. Data supporting alternate specimen types and freeze-thaw antibody stability is lacking. The stability of IgG and other immunoglobulins in multiple blood sample types stored in differing conditions and multiple freeze-thaw cycles (FTCs) was evaluated.

Materials and methods

Serum, plasma, and heparinized-plasma samples were collected from COVID-19 recovered individuals. Samples underwent testing for SARS-CoV-2 antibodies upon collection, after each of 10-12 FTCs, and storage at -70°C, -20°C, 4°C, and room-temperature for 10-12 days using four high-throughput commercial assays, two rapid-test cassettes, a manual ELISA, and a surrogate neutralization assay.

Results

All three spec

SUBMITTER: Kanji JN 

PROVIDER: S-EPMC8310571 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature