A cell engineering approach to enzyme-based fed-batch fermentation.

ABSTRACT:

Background

A fundamental problem associated with E. coli fermentations is the difficulty in achieving high cell densities in batch cultures, attributed in large part to the production and accumulation of acetate through a phenomenon known as overflow metabolism when supplying enough glucose for the cell density desired. Although a fed-batch configuration is the standard method for reducing such issues, traditional fed-batch systems require components which become problematic when applying them at smaller scale. One alternative has been the development of a system whereby the enzymatic degradation of starch is used to release glucose at a controlled rate. However, to date, amylolytic enzymes have only been applied to the culture exogenously, whereas our goal is to design and constru

SUBMITTER: Sibley M 

PROVIDER: S-EPMC8310608 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature