Antibody-Free Mass Spectrometry Identification of Vascular Integrity Markers in Major Trauma.

ABSTRACT: Antibody mediated strategies for protein biomarker detection are common, but may limit discovery. We hypothesized that the use of antibody-free proteomics is feasible for detecting protein biomarkers in plasma of patients sustaining major trauma. A subset of subjects with major trauma from a prospective observational trial were analyzed. Patients were assigned to one of four groups based on their presenting Abbreviated Injury Severity Score (AIS). Sensitive, antibody-free selective reaction monitoring (SRM) mass spectrometry (MS), with spiked-in isotopically labeled synthetic peptides, was used for targeted protein quantification of a panel of 10 prospective targets. An overall tiered sensitivity analytical approach was used for peptide detection and quantification based upon plasma immuno

SUBMITTER: Hinson HE 

PROVIDER: S-EPMC8310742 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature