Single-cell atlas of hepatic T cells reveals expansion of liver-resident naive-like CD4⁺ T cells in primary sclerosing cholangitis.

ABSTRACT:

Background & aims

Little is known about the composition of intrahepatic immune cells and their contribution to the pathogenesis of primary sclerosing cholangitis (PSC). Herein, we aimed to create an atlas of intrahepatic T cells and thereby perform an in-depth characterization of T cells in inflamed human liver.

Methods

Different single-cell RNA sequencing methods were combined with in silico analyses on intrahepatic and peripheral T cells from patients with PSC (n = 11) and healthy donors (HDs, n = 4). Multi-parameter flow cytometry and functional in vitro experiments were conducted on samples from patients with PSC (n = 24), controls with other liver diseases and HDs.

Results

We identified a population of intrahepatic naive-like CD4⁺ T cells, which was pr

SUBMITTER: Poch T 

PROVIDER: S-EPMC8310924 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature