An investigation of alkaline phosphatase enzymatic activity after electrospinning and electrospraying.

ABSTRACT: The high target specificity and multifunctionality of proteins has led to great interest in their clinical use. To this end, the development of delivery systems capable of preserving their bioactivity and improving bioavailability is pivotal to achieve high effectiveness and satisfactory therapeutic outcomes. Electrohydrodynamic (EHD) techniques, namely electrospinning and electrospraying, have been widely explored for protein encapsulation and delivery. In this work, monoaxial and coaxial electrospinning and electrospraying were used to encapsulate alkaline phosphatase (ALP) into poly(ethylene oxide) fibres and particles, respectively, and the effects of the processing techniques on the integrity and bioactivity of the enzyme were assessed. A full morphological and physicochemical charact

SUBMITTER: Onyekuru LC 

PROVIDER: S-EPMC8312041 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature