Determination of Silver Nanoparticle Dose in vitro.

ABSTRACT: An important issue for interpreting in vitro nanomaterial testing is quantifying the dose delivered to target cells. Considerations include the concentration added to the culture, the proportion of the applied dose that interacts with the target cells, and the amount that is eventually absorbed by the target cells. Rapid and efficient techniques are needed to determine delivered doses. Previously, we demonstrated that TiO₂ and silver nanoparticles (AgNP) were absorbed by cells in a dose dependent manner between 1 μg/ml and 30 μg/ml and were detected in cells by light scatter using a flow cytometer. Here, we compare four potential indices of the dose of AgNP to cells, including: inductively coupled plasma - mass spectrometry (ICP-MS); flow cytometry side scatter (SSC); and

SUBMITTER: Ortenzio J 

PROVIDER: S-EPMC8312577 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature