New targets acquired: Improving locus recovery from the Angiosperms353 probe set.

ABSTRACT:

Premise

Universal target enrichment kits maximize utility across wide evolutionary breadth while minimizing the number of baits required to create a cost-efficient kit. The Angiosperms353 kit has been successfully used to capture loci throughout the angiosperms, but the default target reference file includes sequence information from only 6-18 taxa per locus. Consequently, reads sequenced from on-target DNA molecules may fail to map to references, resulting in fewer on-target reads for assembly, and reducing locus recovery.

Methods

We expanded the Angiosperms353 target file, incorporating sequences from 566 transcriptomes to produce a 'mega353' target file, with each locus represented by 17-373 taxa. This mega353 file is a drop-in replacement for the original Angiosperms353

SUBMITTER: McLay TGB 

PROVIDER: S-EPMC8312740 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature