Target sequence capture in orchids: Developing a kit to sequence hundreds of single-copy loci.

ABSTRACT:

Premise

Understanding relationships among orchid species and populations is of critical importance for orchid conservation. Target sequence capture has become a standard method for extracting hundreds of orthologous loci for phylogenomics. Up-front cost and time associated with design of bait sets makes this method prohibitively expensive for many researchers. Therefore, we designed a target capture kit to reliably sequence hundreds of orthologous loci across orchid lineages.

Methods

We designed an Orchidaceae target capture bait set for 963 single-copy genes identified in published orchid genome sequences. The bait set was tested on 28 orchid species, with representatives of the subfamilies Cypripedioideae, Orchidoideae, and Epidendroideae.

Results

Between 1,518,041

SUBMITTER: Eserman LA 

PROVIDER: S-EPMC8312744 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature