On the potential of Angiosperms353 for population genomic studies.

ABSTRACT:

Premise

The successful application of universal targeted sequencing markers, such as those developed for the Angiosperms353 probe set, within populations could reduce or eliminate the need for specific marker development, while retaining the benefits of full-gene sequences in population-level analyses. However, whether the Angiosperms353 markers provide sufficient variation within species to calculate demographic parameters is untested.

Methods

Using herbarium specimens from a 50-year-old floristic survey in Texas, we sequenced 95 samples from 24 species using the Angiosperms353 probe set. Our data workflow calls variants within species and prepares data for population genetic analysis using standard metrics. In our case study, gene recovery was affected by genomic library c

SUBMITTER: Slimp M 

PROVIDER: S-EPMC8312745 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature