Rapid Formation of Polymer Frits in Fused Silica Capillaries Using Spatially defined Thermal Free-Radical Initiated Polymerization.

ABSTRACT: Column preparation in capillary chromatography commonly relies upon the generation of on-column porous frits. Here, we report a simple, robust and low-cost approach for preparing polymer frits on-column, in a rapid and spatially controlled manner using thermal free-radical initiated polymerization. In this approach, a simple, temperature-controlled heating apparatus is positioned adjacent to a 100 μm i.d. fused-silica capillary for a defined duration. Frits were synthesized in 3-(trimethoxysilyl)propyl methacrylate modified capillaries using a monomer solution of 2,2-azobisisobutyronitrile, glycidyl methacrylate, ethylene glycol dimethacrylate, and decanol. Frit length and stability were investigated as a function of polymerization time and temperature. Frit length was easily contro

SUBMITTER: Sandy KE 

PROVIDER: S-EPMC8313020 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature