A piggyBac-mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice.

ABSTRACT: Targeted mutagenesis via CRISPR/Cas9 is now widely used, not only in model plants but also in agriculturally important crops. However, in vegetative crop propagation, CRISPR/Cas9 expression cassettes cannot be segregated out in the resulting progenies, but must nevertheless be eliminated without leaving unnecessary sequences in the genome. To this end, we designed a piggyBac-mediated transgenesis system for the temporary expression of CRISPR/Cas9 in plants. This system allows integration into the host genome of piggyBac carrying both CRISPR/Cas9 and positive selection marker expression cassettes from an extrachromosomal double-stranded transfer DNA (dsT-DNA), with subsequent excision of the transgenes by the re-transposition of piggyBac from the host genome after successful induction of ta

SUBMITTER: Nishizawa-Yokoi A 

PROVIDER: S-EPMC8313132 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature