Development and application of an efficient recombineering system for Burkholderia glumae and Burkholderia plantarii.

ABSTRACT: The lambda phage Red proteins Redα/Redβ/Redγ and Rac prophage RecE/RecT proteins are powerful tools for precise and efficient genetic manipulation but have been limited to only a few prokaryotes. Here, we report the development and application of a new recombineering system for Burkholderia glumae and Burkholderia plantarii based on three Rac bacteriophage RecET-like operons, RecEThe_BDU8 , RecETh_TJI49 and RecETh1h2e_YI23 , which were obtained from three different Burkholderia species. Recombineering experiments indicated that RecETh_TJI49 and RecETh1h2e_YI23 showed higher recombination efficiency compared to RecEThe_BDU8 in Burkholderia glumae PG1. Furthermore, all of the proteins currently categorized as hypothetical proteins i

SUBMITTER: Li R 

PROVIDER: S-EPMC8313284 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature