Recombinant protein secretion by Bacillus subtilis and Lactococcus lactis: pathways, applications, and innovation potential.

ABSTRACT: Secreted recombinant proteins are of great significance for industry, healthcare and a sustainable bio-based economy. Consequently, there is an ever-increasing need for efficient production platforms to deliver such proteins in high amounts and high quality. Gram-positive bacteria, particularly bacilli such as Bacillus subtilis, are favored for the production of secreted industrial enzymes. Nevertheless, recombinant protein production in the B. subtilis cell factory can be very challenging due to bottlenecks in the general (Sec) secretion pathway as well as this bacterium's intrinsic capability to secrete a cocktail of highly potent proteases. This has placed another Gram-positive bacterium, Lactococcus lactis, in the focus of attention as an alternative, non-proteolytic, cell factory for

SUBMITTER: Neef J 

PROVIDER: S-EPMC8314018 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature