Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways.

ABSTRACT: Biotechnological production in bacteria enables access to numerous valuable chemical compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well as the combinatorial use of biocatalysts, pathways, and circuits even bring new-to-nature compounds within reach. However, the associated substrates and biosynthetic products often cause severe chemical stress to the bacterial hosts. Species of the Pseudomonas clade thus represent especially valuable chassis as they are endowed with multiple stress response mechanisms, which allow them to cope with a variety of harmful chemicals. A built-in cell envelope stress response enables fast adaptations that sustain membrane integrity under adverse conditions. Further, effective export machineries can prevent intracellular accumula

SUBMITTER: Bitzenhofer NL 

PROVIDER: S-EPMC8314020 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature