Reverse transcription priming methods affect normalisation choices for gene expression levels in oocytes and early embryos.

ABSTRACT: Mammalian oocytes and embryos rely exclusively on maternal mRNAs to accomplish early developmental processes. Since oocytes and early embryos are transcriptionally silent after meiotic resumption, most of the synthesised maternal mRNA does not undergo immediate translation but is instead stored in the oocyte. Quantitative RT-PCR is commonly used to quantify mRNA levels, and correct quantification relies on reverse transcription and the choice of reference genes. Different methods for reverse transcription may affect gene expression determination in oocytes. In this study, we examined the suitability of either random or oligo(dT) primers for reverse transcription to be used for quantitative RT-PCR. We further looked for changes in poly(A) length of the maternal mRNAs during oocyte maturatio

SUBMITTER: Yu B 

PROVIDER: S-EPMC8314208 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature