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DeTOKI identifies and characterizes the dynamics of chromatin TAD-like domains in a single cell.

ABSTRACT: Topologically associating domains (TAD) are a key structure of the 3D mammalian genomes. However, the prevalence and dynamics of TAD-like domains in single cells remain elusive. Here we develop a new algorithm, named deTOKI, to decode TAD-like domains with single-cell Hi-C data. By non-negative matrix factorization, deTOKI seeks regions that insulate the genome into blocks with minimal chance of clustering. deTOKI outperforms competing tools and reliably identifies TAD-like domains in single cells. Finally, we find that TAD-like domains are not only prevalent, but also subject to tight regulation in single cells.

SUBMITTER: Li X

PROVIDER: S-EPMC8314462 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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DeTOKI identifies and characterizes the dynamics of chromatin TAD-like domains in a single cell.

Li Xiao X Zeng Guangjie G Li Angsheng A Zhang Zhihua Z

Genome biology 20210727 1

Topologically associating domains (TAD) are a key structure of the 3D mammalian genomes. However, the prevalence and dynamics of TAD-like domains in single cells remain elusive. Here we develop a new algorithm, named deTOKI, to decode TAD-like domains with single-cell Hi-C data. By non-negative matrix factorization, deTOKI seeks regions that insulate the genome into blocks with minimal chance of clustering. deTOKI outperforms competing tools and reliably identifies TAD-like domains in single cel ...[more]

PMID: 34311744

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