Engineering of Saccharomyces cerevisiae for the accumulation of high amounts of triacylglycerol.

ABSTRACT:

Background

Fatty acid-based substances play an important role in many products, from food supplements to pharmaceutical products and biofuels. The production of fatty acids, mainly in their esterified form as triacylglycerol (TAG), has been intensively studied in oleaginous yeasts, whereas much less effort has been invested into non-oleaginous species. In the present work, we engineered the model yeast Saccharomyces cerevisiae, which is commonly regarded as non-oleaginous, for the storage of high amounts of TAG, comparable to the contents achieved in oleaginous yeasts.

Results

We investigated the effects of several mutations with regard to increased TAG accumulation and identified six of them as important for this phenotype: a point mutation in the acetyl-CoA carboxylase Acc

SUBMITTER: Arhar S 

PROVIDER: S-EPMC8314538 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature