Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices.

ABSTRACT: Live pancreatic tissue slices allow for the study of islet physiology and function in the context of an intact islet microenvironment. Slices are prepared from live human and mouse pancreatic tissue embedded in agarose and cut using a vibratome. This method allows for the tissue to maintain viability and function in addition to preserving underlying pathologies such as type 1 (T1D) and type 2 diabetes (T2D). The slice method enables new directions in the study of the pancreas through the maintenance of the complex structures and various intercellular interactions that comprise the endocrine and exocrine tissues of the pancreas. This protocol demonstrates how to perform staining and time-lapse microscopy of live endogenous immune cells within pancreatic slices along with assessments of isle

SUBMITTER: Huber MK 

PROVIDER: S-EPMC8314551 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature