Comparative iTRAQ proteomics identified proteins associated with sperm maturation between yak and cattleyak epididymis.

ABSTRACT:

Background

During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we employed iTRAQ proteomics to investigate proteins associated with sperm maturation between yak and cattleyak epididymis.

Results

After a successful sampling and protein extraction, the iTRAQ coupled with LC-MS/MS mass spectrometry and bioinformatics analysis were performed. We identified 288 differentially abundant proteins (DAPs) between yak and cattleyak epididymis; 151 were up-regulat

SUBMITTER: Zhao W 

PROVIDER: S-EPMC8314601 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature