Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs) provide a promising cell source for retinal cell replacement therapy but often lack standardized cell production and live-cell shipment logistics as well as rigorous analyses of surgical procedures for cell transplantation in the delicate macula area. We have previously established a xeno- and feeder cell-free production system for hPSC differentiated retinal pigment epithelial (RPE) cells, and herein, a novel immunosuppressed non-human primate (NHP) model with a disrupted ocular immune privilege is presented for transplanting human embryonic stem cell (hESC)-derived RPE on a scaffold, and the safety and submacular graft integration are assessed. Furthermore, the feasibility of intercontinental shipment of live hESC-RPE is examined.

SUBMITTER: Liu Z 

PROVIDER: S-EPMC8314642 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature