Silencing of METTL3 effectively hinders invasion and metastasis of prostate cancer cells.

ABSTRACT: Background: Since primary prostate cancer (PCa) can advance to the life-threatening metastatic PCa, exploring the molecular mechanisms underlying PCa metastasis is crucial for developing the novel targeted preventive strategies for decreasing the mortality of PCa. RNA N⁶-methyladenosine (m⁶A) is an emerging regulatory mechanism for gene expression and its specific roles in PCa progression remains elusive. Methods: Western blotting, quantitative real-time PCR and immunohistochemical analyses were used to detect target gene expression in PCa cells in vitro and prostate tissues from patients. RNA immunoprecipitation was conducted to analyze the specific binding of mRNA to the target protein. Migration and invasion assays were used to assess the migra

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8315076 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature