Molecular imaging of fibroblast activation protein after myocardial infarction using the novel radiotracer [⁶⁸Ga]MHLL1.

ABSTRACT: Background: Myocardial infarction (MI) evokes an organized remodeling process characterized by the activation and transdifferentiation of quiescent cardiac fibroblasts to generate a stable collagen rich scar. Early fibroblast activation may be amenable to targeted therapy, but is challenging to identify in vivo. We aimed to non-invasively image active fibrosis by targeting the fibroblast activation protein (FAP) expressed by activated (myo)fibroblasts, using a novel positron emission tomography (PET) radioligand [⁶⁸Ga]MHLL1 after acute MI. Methods: One-step chemical synthesis and manual as well as module-based radiolabeling yielded [⁶⁸Ga]MHLL1. Binding characteristics were evaluated in murine and human FAP-transfected cells, and stability tested i

SUBMITTER: Langer LBN 

PROVIDER: S-EPMC8315078 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature