Molecular Insights into the Copper-Sensitive Operon Repressor in Acidithiobacillus caldus.

ABSTRACT: The copper-sensitive operon repressor (CsoR) family, which is the main Cu(I)-sensing family, is widely distributed and regulates regulons involved in detoxification in response to extreme copper stress (a general range of ≥3 g/liter copper ions). Here, we identified CsoR in hyper-copper-resistant Acidithiobacillus caldus (CsoR^Ac), an organism used in the bioleaching process of copper ores. CsoR^Ac possesses highly conserved Cu(I) ligands and structures within the CsoR family members. Transcriptional analysis assays indicated that the promoter (PIII) of csoR was active but weakly responsive to copper in Escherichia coli. Copper titration assays gave a stoichiometry of 0.8 mol Cu(I) per apo-CsoR^Ac monomer in vitro combined with atomic absorption

SUBMITTER: Hou S 

PROVIDER: S-EPMC8315176 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature