G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.
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ABSTRACT:
SUBMITTER: Gao J
PROVIDER: S-EPMC8315639 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.
Gao Jun J Gao Zhaofeng Z Putnam Andrea A AA Byrd Alicia K AK Venus Sarah L SL Marecki John C JC Edwards Andrea D AD Lowe Haley M HM Jankowsky Eckhard E Raney Kevin D KD
Chemical communications (Cambridge, England) 20210701 60
G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid-liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains. ...[more]
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