Methionine Protects Mammary Cells against Oxidative Stress through Producing S-Adenosylmethionine to Maintain mTORC1 Signaling Activity.

ABSTRACT: The mechanistic target of rapamycin complex 1 (mTORC1) signaling plays pivotal roles in cell growth and diseases. However, it remains mechanistically unclear about how to maintain mTORC1 activity during mammary glands development. Here we showed that mammary glands suffered from aggravated oxidative stress as pregnancy advanced and was accompanied by an increase in H₂O₂ levels, while the consumption for methionine and S-adenosylmethionine (SAM) rather than S-adenosylhomocysteine (SAH) were promoted in vivo. Likewise, H₂O₂ promoted SAM synthesis and reduced SAM utilization for methylation depending on H₂O₂ levels and treatment time in vitro. H₂O₂ inhibited phosphorylation of S

SUBMITTER: Zhong H 

PROVIDER: S-EPMC8315855 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature