The Selenophosphate Synthetase Gene, selD, Is Important for Clostridioides difficile Physiology.

ABSTRACT: The endospore-forming pathogen Clostridioides difficile is the leading cause of antibiotic-associated diarrhea and is a significant burden on the community and health care. C. difficile, like all forms of life, incorporates selenium into proteins through a selenocysteine synthesis pathway. The known selenoproteins in C. difficile are involved in a metabolic process that uses amino acids as the sole carbon and nitrogen source (Stickland metabolism). The Stickland metabolic pathway requires the use of two selenium-containing reductases. In this study, we built upon our initial characterization of the CRISPR-Cas9-generated selD mutant by creating a CRISPR-Cas9-mediated restoration of the selD gene at the native locus. Here, we use these CRISPR-generated strains to analyze the im

SUBMITTER: McAllister KN 

PROVIDER: S-EPMC8315937 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature