Chromatin Accessibility of Human Mitral Valves and Functional Assessment of MVP Risk Loci.

ABSTRACT:

Rationale

Mitral valve prolapse (MVP) is a common valvopathy that leads to mitral insufficiency, heart failure, and sudden death. Functional genomic studies in mitral valves are needed to better characterize MVP-associated variants and target genes.

Objective

To establish the chromatin accessibility profiles and assess functionality of variants and narrow down target genes at MVP loci.

Methods and results

We mapped the open chromatin regions in nuclei from 11 human pathogenic and 7 nonpathogenic mitral valves by an assay for transposase-accessible chromatin with high-throughput sequencing. Open chromatin peaks were globally similar between pathogenic and nonpathogenic valves. Compared with the heart tissue and cardiac fibroblasts, we found that MV-specific assay for t

SUBMITTER: Kyryachenko S 

PROVIDER: S-EPMC8316483 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature