Role of Serosal TRPV4-Constituted SOCE Mechanism in Secretagogues-Stimulated Intestinal Epithelial Anion Secretion.

ABSTRACT: As little is known about the role of calcium (Ca²⁺) signaling mediating the small intestinal epithelial anion secretion, we aimed to study its regulatory role in secretagogue-stimulated duodenal anion secretion and the underlying molecular mechanisms. Therefore, intestinal anion secretion from native mouse duodenal epithelia was examined with Ussing chambers to monitor PGE₂-, 5-HT-, and CCh-induced short-circuit currents (I _sc ). PGE₂ (10 μM) and 5-HT (10 μM) induced mouse duodenal I _sc , markedly attenuated by serosal Ca²⁺-free solution and selective blockers of store-operated Ca²⁺ channels on the serosal side of the duodenum. Furthermore, PGE₂- and 5-HT-induced duodenal

SUBMITTER: Cui Y 

PROVIDER: S-EPMC8317263 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature