Spatial epi-proteomics enabled by histone post-translational modification analysis from low-abundance clinical samples.

ABSTRACT:

Background

Increasing evidence linking epigenetic mechanisms and different diseases, including cancer, has prompted in the last 15 years the investigation of histone post-translational modifications (PTMs) in clinical samples. Methods allowing the isolation of histones from patient samples followed by the accurate and comprehensive quantification of their PTMs by mass spectrometry (MS) have been developed. However, the applicability of these methods is limited by the requirement for substantial amounts of material.

Results

To address this issue, in this study we streamlined the protein extraction procedure from low-amount clinical samples and tested and implemented different in-gel digestion strategies, obtaining a protocol that allows the MS-based analysis of the most commo

SUBMITTER: Noberini R 

PROVIDER: S-EPMC8317427 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature