Norswertianolin Promotes Cystathionine γ-Lyase Activity and Attenuates Renal Ischemia/Reperfusion Injury and Hypertension.

ABSTRACT: Cystathionine gamma-lyase (CSE)/hydrogen sulfide (H₂S) plays a protective role in cardiovascular diseases including hypertension and ischemia/reperfusion (I/R) injury. This study was aimed to screen natural small molecule compounds that activate CSE activity and then evaluate its effect(s) on kidney I/R injury and hypertension. Applying computer molecular docking technology, we screened the natural small molecule compound norswertianolin (NW)-specific binding to CSE. Using the microscale thermophoresis technology, we confirmed that the Leu68 site was the essential hydrogen bond site of NW binding to CSE. NW supplementation significantly increased CSE expression and its activity for H₂S generation both in vivo and in vitro. In the model of acute and long-

SUBMITTER: Niu Y 

PROVIDER: S-EPMC8317460 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature