Analysis of Prostate Cancer Tumor Microenvironment Identifies Reduced Stromal CD4 Effector T-cell Infiltration in Tumors with Pelvic Nodal Metastasis.

ABSTRACT:

Background

Pelvic nodal metastasis in prostate cancer impacts patient outcome negatively.

Objective

To explore tumor-infiltrating immune cells as a potential predictive tool for regional lymph node (LN) metastasis.

Design setting and participants

We applied multiplex immunofluorescence and targeted transcriptomic analysis on 94 radical prostatectomy specimens in patients with (LN+) or without (LN-) pelvic nodal metastases. Both intraepithelial and stromal infiltrations of immune cells and differentially expressed genes (mRNA and protein levels) were correlated with the nodal status.

Outcome measurements and statistical analysis

The identified CD4 effector cell signature of nodal metastasis was validated in a comparable independent patient cohort of 184 informat

SUBMITTER: Ntala C 

PROVIDER: S-EPMC8317840 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature