Melatonin mitigates the adverse effect of hypoxia during myocardial differentiation in mouse embryonic stem cells.

ABSTRACT:

Background

Hypoxia causes oxidative stress and affects cardiovascular function and the programming of cardiovascular disease. Melatonin promotes antioxidant enzymes such as superoxide dismutase, glutathione reductase, glutathione peroxidase, and catalase.

Objectives

This study aims to investigate the correlation between melatonin and hypoxia induction in cardiomyocytes differentiation.

Methods

Mouse embryonic stem cells (mESCs) were induced to myocardial differentiation. To demonstrate the influence of melatonin under hypoxia, mESC was pretreated with melatonin and then cultured in hypoxic condition. The cardiac beating ratio of the mESC-derived cardiomyocytes, mRNA and protein expression levels were investigated.

Results

Under hypoxic condition, the mRNA expre

SUBMITTER: Lee JH 

PROVIDER: S-EPMC8318788 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature