Induction of antigen-specific Treg cells in treating autoimmune uveitis via bystander suppressive pathways without compromising anti-tumor immunity.

ABSTRACT:

Background

Induction of autoantigen-specific Treg cells that suppress tissue-specific autoimmunity without compromising beneficial immune responses is the holy-grail for immunotherapy to autoimmune diseases.

Methods

In a model of experimental autoimmune uveitis (EAU) that mimics human uveitis, ocular inflammation was induced by immunization with retinal antigen interphotoreceptor retinoid-binding protein (IRBP). Mice were given intraperitoneal injection of αCD4 antibody (Ab) after the onset of disease, followed by administration of IRBP. EAU was evaluated clinically and functionally. Splenocytes, CD4⁺CD25^- and CD4⁺CD25⁺ T cells were sorted and cultured with IRBP or αCD3 Ab. T cell proliferation and cytokine production were assessed

SUBMITTER: Chen Z 

PROVIDER: S-EPMC8318874 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature