Differentiated activities of decorin and biglycan in the progression of post-traumatic osteoarthritis.

ABSTRACT:

Objective

To delineate the activities of decorin and biglycan in the progression of post-traumatic osteoarthritis (PTOA).

Design

Three-month-old inducible biglycan (Bgn^iKO) and decorin/biglycan compound (Dcn/Bgn^iKO) knockout mice were subjected to the destabilization of the medial meniscus (DMM) surgery to induce PTOA. The OA phenotype was evaluated by assessing joint structure and sulfated glycosaminoglycan (sGAG) staining via histology, surface collagen fibril nanostructure and calcium content via scanning electron microscopy, tissue modulus via atomic force microscopy-nanoindentation, as well as subchondral bone structure and meniscus ossification via micro-computed tomography. Outcomes were compared with previous findings in the inducible decorin (D

SUBMITTER: Han B 

PROVIDER: S-EPMC8319061 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature