A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.

ABSTRACT: We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.

SUBMITTER: Strobl F 

PROVIDER: S-EPMC8319312 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature