HSP60 reduction protects against diet-induced obesity by modulating energy metabolism in adipose tissue.

ABSTRACT:

Objective

Insulin regulates mitochondrial function, thereby propagating an efficient metabolism. Conversely, diabetes and insulin resistance are linked to mitochondrial dysfunction with a decreased expression of the mitochondrial chaperone HSP60. The aim of this investigation was to determine the effect of a reduced HSP60 expression on the development of obesity and insulin resistance.

Methods

Control and heterozygous whole-body HSP60 knockout (Hsp60^+/-) mice were fed a high-fat diet (HFD, 60% calories from fat) for 16 weeks and subjected to extensive metabolic phenotyping. To understand the effect of HSP60 on white adipose tissue, microarray analysis of gonadal WAT was performed, ex vivo experiments were performed, and a lentiviral knockdown of HSP60 in 3T3-L1 ce

SUBMITTER: Hauffe R 

PROVIDER: S-EPMC8319365 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature