A microfluidic array device for single cell capture and intracellular Ca2+ response analysis induced by dynamic biochemical stimulus.

ABSTRACT: A microfluidic array was constructed for trapping single cell and loading identical dynamic biochemical stimulation for gain a better understanding of Ca2+ signaling at single cell resolution in the present study. This microfluidic array consists of multiple radially aligned flow channels with equal intersection angles, which was designed by a combination of stagnation point flow and physical barrier. Numerical simulation results and trajectory analysis have shown the effectiveness of this single cell trapping device. Fluorescent experiment results demonstrated the effects of flow rate and frequency of dynamic stimulus on the profiles of biochemical concentration which exposed on captured cells. In this microarray, the captured single cells in each trapping channels were able to receive id

SUBMITTER: Wei W 

PROVIDER: S-EPMC8319492 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature