Fluorescent Probes with Unnatural Amino Acids to Monitor Proteasome Activity in Real-Time.

ABSTRACT: The proteasome is an essential protein complex that, when dysregulated, can result in various diseases in eukaryotic cells. As such, understanding the enzymatic activity of the proteasome and what can alter it is crucial to elucidating its roles in these diseases. This can be done effectively by using activity-based fluorescent substrate probes, of which there are many commercially available that target the individual protease-like subunits in the 20S CP of the proteasome. Unfortunately, these probes have not displayed appropriate characteristics for their use in live cell-based assays. In the work presented here, we have developed a set of probes which have shown improved fluorescence properties and selectivity toward the proteasome compared to other cellular proteases. By including unnat

SUBMITTER: Zerfas BL 

PROVIDER: S-EPMC8319958 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature