Direct identification of neoantigen-specific TCRs from tumor specimens by high-throughput single-cell sequencing.

ABSTRACT:

Background

Recognition of neoantigens by T cells plays a major role in cancer immunotherapy. Identification of neoantigen-specific T-cell receptors (TCRs) has become a critical research tool for studying T cell-mediated responses after immunotherapy. In addition, neoantigen-specific TCRs can be used to modify the specificity of T cells for T cell-based therapies targeting tumor-specific mutations. Although several techniques have been developed to identify TCR sequences, these techniques still require a significant amount of labor, making them impractical in the clinical setting.

Methods

Thanks to the availability of high-throughput single-cell sequencing, we developed a new process to isolate neoantigen-specific TCR sequences. This process included the isolation of tumor-in

SUBMITTER: Lu YC 

PROVIDER: S-EPMC8320258 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature