Using yeast two-hybrid system and molecular dynamics simulation to detect venom protein-protein interactions.

ABSTRACT: Proteins and peptides are major components of snake venom. Venom protein transcriptomes and proteomes of many snake species have been reported; however, snake venom complexity (i.e., the venom protein-protein interactions, PPIs) remains largely unknown. To detect the venom protein interactions, we used the most common snake venom component, phospholipase A₂s (PLA₂s) as a "bait" to identify the interactions between PLA₂s and 14 of the most common proteins in Western diamondback rattlesnake (Crotalus atrox) venom by using yeast two-hybrid (Y2H) analysis, a technique used to detect PPIs. As a result, we identified PLA₂s interacting with themselves, and lysing-49 PLA₂ (Lys49 PLA₂) interacting with venom cysteine-rich secr

SUBMITTER: Jia Y 

PROVIDER: S-EPMC8320608 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature