In Situ Generated Novel ¹H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells.

ABSTRACT: For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive ¹H-MRI probe to induce significant ¹H-MRI contrast changes in relaxation times T ₁ and T ₂ in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe³⁺. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 br

SUBMITTER: Gao S 

PROVIDER: S-EPMC8321238 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature