Cell-Type-Specific High Throughput Toxicity Testing in Human Midbrain Organoids.

ABSTRACT: Toxicity testing is a crucial step in the development and approval of chemical compounds for human contact and consumption. However, existing model systems often fall short in their prediction of human toxicity in vivo because they may not sufficiently recapitulate human physiology. The complexity of three-dimensional (3D) human organ-like cell culture systems ("organoids") can generate potentially more relevant models of human physiology and disease, including toxicity predictions. However, so far, the inherent biological heterogeneity and cumbersome generation and analysis of organoids has rendered efficient, unbiased, high throughput evaluation of toxic effects in these systems challenging. Recent advances in both standardization and quantitative fluorescent imaging enabled us to

SUBMITTER: Renner H 

PROVIDER: S-EPMC8321240 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature