Imaging Meets Cytometry: Analyzing Heterogeneous Functional Microscopic Data from Living Cell Populations.

ABSTRACT: Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships between islet cell populations. We therefore believe tha

SUBMITTER: Draper M 

PROVIDER: S-EPMC8321243 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature