Transcriptome Analysis in Yeast Reveals the Externality of Position Effects.

ABSTRACT: The activity of a gene newly integrated into a chromosome depends on the genomic context of the integration site. This "position effect" has been widely reported, although the other side of the coin, that is, how integration affects the local chromosomal environment, has remained largely unexplored, as have the mechanism and phenotypic consequences of this "externality" of the position effect. Here, we examined the transcriptome profiles of approximately 250 Saccharomyces cerevisiae strains, each with GFP integrated into a different locus of the wild-type strain. We found that in genomic regions enriched in essential genes, GFP expression tended to be lower, and the genes near the integration site tended to show greater expression reduction. Further joint analysis with public genome-wide h

SUBMITTER: Gui Q 

PROVIDER: S-EPMC8321525 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature