Exploring a Local Genetic Interaction Network Using Evolutionary Replay Experiments.

ABSTRACT: Understanding how genes interact is a central challenge in biology. Experimental evolution provides a useful, but underutilized, tool for identifying genetic interactions, particularly those that involve non-loss-of-function mutations or mutations in essential genes. We previously identified a strong positive genetic interaction between specific mutations in KEL1 (P344T) and HSL7 (A695fs) that arose in an experimentally evolved Saccharomyces cerevisiae population. Because this genetic interaction is not phenocopied by gene deletion, it was previously unknown. Using "evolutionary replay" experiments, we identified additional mutations that have positive genetic interactions with the kel1-P344T mutation. We replayed the evolution of this population 672 times from six timepoints. We identifie

SUBMITTER: Vignogna RC 

PROVIDER: S-EPMC8321538 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature